Extended Data Figure 5: Expression of CSNK1A1 and IKZF1 in patient samples.

a, mRNA expression of CSNK1A1 in cord blood CD34⁺ cells infected with lentivirus expressing human CSNK1A1 or empty vector. CD34⁺ cells were infected with GFP-tagged lentivirus and GFP⁺ cells were sorted three days later. Values are mean ± s.d., n = 4 biological replicates, each with 3 technical replicates. b, mRNA expression of IKZF1 in cord blood CD34⁺ cells infected with lentivirus expressing IKZF1 or empty vector as in a. Values are mean ± s.d., n = 3 biological replicates, each with 3 technical replicates. c, CD34⁺ cells derived from patient or control bone marrow were transduced with a lentivirus expressing human IKZF1 (hIKZF1) and GFP or an empty control vector and treated with DMSO or 1 µM lenalidomide. The percentage of GFP⁺ cells was assessed by flow cytometry after five days for each vector-drug combination. Results are reported as a ratio of the percentage of GFP⁺ cells in the lenalidomide condition to the percentage of GFP⁺ cells in the DMSO condition. Results are combined from three experiments. d, Characteristics of patient samples used for CSNK1A1 and IKZF1 expression experiments. Results of TP53 sequencing, including exons with adequate coverage, is given in the rightmost column. All samples sequenced had wild-type TP53. ND, not done due to limited patient material. WT, TP53 exon sequence has only known benign polymorphisms.