Extended Data Figure 8: Comparison of the effects of thalidomide derivatives.
From: Lenalidomide induces ubiquitination and degradation of CK1α in del(5q) MDS
a, Comparison of log2 ratios for CK1α and IKZF1 in MDS-L cells after treatment with lenalidomide or CC-122 for 24 or 72 h assessed by tandem mass tag (TMT) quantitative proteomics. Analysis was performed with n = 4 for DMSO control and n = 3 for each drug treatment time point. b, Adjusted P values for CK1α and IKZF1 proteomic data in MDS-L cells. c, Western blot validation of IKZF1 and CK1α levels in DMSO (n = 4), lenalidomide (n = 3) and CC-122 (n = 3) treated samples used for MDS-L proteomic analysis. d, Western blot validation of the effects of the different agents on CK1α and IKZF1 protein levels in KG-1 cells. e, Effect of lenalidomide, pomalidomide (Pom), and thalidomide (Thal) on protein levels of CK1α, β-catenin, and IKZF1 in KG-1 cells treated for 24 h with the indicated drug concentrations. f, Effect of CC-122 and lenalidomide on β-catenin protein levels in KG-1 cells after 72 h. g, Effect of lenalidomide on CK1α and β-catenin protein levels in HEL cells. Data are representative of two (e, g) or three (c, d) independent experiments. Uncropped blots are shown in Supplementary Fig. 1.
