Extended Data Figure 4: Endogenous expression of newly described reporter genes in hatched Ciona larvae. | Nature

Extended Data Figure 4: Endogenous expression of newly described reporter genes in hatched Ciona larvae.

From: The pre-vertebrate origins of neurogenic placodes

Extended Data Figure 4

a–d, Bright field anterior lateral views. a, Animal hybridized with a GnRH2 mRNA probe. White arrowhead indicates the position of the oral opening. b, Animal hybridized with an RXFP3 mRNA probe. c, Animal hybridized with a SOG/Chemokine-like mRNA probe. The heavily stained tunic was manually removed to reveal the expression signal. d, Animal hybridized with a CNGA mRNA probe. Red arrows mark areas of comparable expression throughout the panels in presumed PPE-derived neurons. An adjacent signal in the ocellus-associated photoreceptors makes it difficult to discriminate expression in PPE-derived neurons in panel d.

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