Extended Data Figure 5: GnRH expression requires BMP attenuation.

a–d, Lateral view of a larva electroporated with GnRH>YFP and counterstained with phalloidin (violet). a, Larva co-electroporated with Dmrt>BMP2/4. Of the 200 larvae, 196 had no GnRH>YFP expression in aATENs and displayed mild to severe morphogenetic defects. Bracket shows mild anterior morphological defect. b, Larva co-electroporated with Dmrt>BMP5/7. Of the 200 larvae, 124 had GnRH>YFP expression in aATENs. c, Larva co-electroporated with Dmrt>BMPR1^CA . Of the 200 larvae, 197 larvae had no GnRH>YFP expression in aATENs. d, Larva co-electroporated with Dmrt>TGF-βR^CA . Of the 200 larvae, 130 had GnRH>YFP expression in aATENs and most displayed severe anterior neural tube defects. Arrowheads in b and d indicate the position of GnRH expression in aATENs. Anterior is to the left in all images.