Extended Data Figure 3: Gating scheme for FACS experiments.
From: PIK3CAH1047R induces multipotency and multi-lineage mammary tumours
a–d, Representative FACS plots of K8-CreERT2/Tomato (a), K8-CreERT2/PIK3CAH1047R/Tomato (b), Lgr5-CreERT2/PIK3CAWT (c) and Lgr5-CreERT2/PIK3CAH1047R (d) animals 4 weeks after tamoxifen injection. The gating strategy shown illustrates the elimination of doublets, dead cells (DAPIHi), and white blood cells (CD45+) and the sorting of Tomato- or GFP-positive mammary epithelial subsets (basal CD24LoSca1−, luminal CD24HiSca1−; luminal CD24HiSca1+).
