Extended Data Figure 1: Measurement of endogenous and exogenous gene expression in D. rerio. | Nature

Extended Data Figure 1: Measurement of endogenous and exogenous gene expression in D. rerio.

From: Mutations in DCHS1 cause mitral valve prolapse

Extended Data Figure 1

a, Corresponding representative embryos of each morpholino knockdown on the left, with close-up of heart on the right. b, To assess efficiency of morpholino knockdown, 20 embryos were collected 72 h after injection, mRNA was collected, and quantitative PCR was performed with three technical replicates. We demonstrate that morpholino (MO) knockdown of each indicated gene results in reduced mRNA expression, after normalization to beta-actin expression, compared to mock-injected controls (two-sided Student’s t-test). P values are noted on graphs. c, Western blotting of 20 pooled embryos injected with DCHS1 mRNA demonstrates the production of protein. Mutant mRNA refers to the compound mutant P197L/R2513H.

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