Extended Data Figure 4: Dachsous1b knockdown alters atrioventricular ring markers.

In situ hybridization at 48 hpf and 72 hpf, as indicated, was performed for known atrioventricular ring markers. In contrast to WT (a) bmp4 expression is expanded into the ventricle at 48 hpf in dchs1 knockdown embryos at 48 hpf (b), spp1 and notch1b expression was largely unperturbed (c–f), and has2 expression was not detected at 48 hpf, and is faint at 72 hpf in dchs1 knockdown, compared to identically handled and stained controls (i–l).