Extended Data Figure 1: The 29-component PIC assembled on SNR20* short promoter.
From: Real-time observation of the initiation of RNA polymerase II transcription
a, PIC excluding the kinase domain (TFIIK) was assembled on SNR20* short (adjacent to the 2.7-kb downstream handle sequence) and sedimented on a glycerol gradient; fractions were analysed by SDS–PAGE. b, The results from fraction 12, annotated in detail, indicate that all PIC components were retained, confirming that the complex reconstituted fully from the component proteins. The subunit(s) of Pol II are labelled in black, TFIIF in blue, TFIIE in magenta, TFIIH in orange, TFIIA in cyan, TFIIB in red, TBP in light green, and Sub1 in dark green. TFIIK (3-subunits) was later added to the PIC.
