Extended Data Figure 9: SEC14L2 expression does not enhance replication of lipid peroxidation-resistant RNA viruses.

a–f, Flaviviruses, such as yellow fever virus (YFV) and dengue virus (DENV) do not respond to SEC14L2 expression. a–e, Empty-vector- and SEC14L2-expressing Huh-7.5 cells were infected with YFV-venus (a, b) or DENV-GFP (d, e) at a multiplicity of infection (MOI) of 0.01 and 0.1, respectively. The cells were harvested at the indicated times post-infection, followed by FACS analysis. a, d, The number of yellow (YFV-venus) and green (DENV-GFP) cells are plotted as percentage of positive cells and b, e, the mean fluorescence intensities (MFI) are presented as percentage relative to empty vector. c, f, Empty-vector- and SEC14L2-expressing Huh-7.5 cells were inoculated with c, YFV (17D) or f, DENV (serotype 2 strain 16681) at an MOI of 0.5 and 0.1, respectively. The culture medium was collected at the indicated times post infection and the infectious virus titres were determined by a plaque formation assay on Huh-7.5 cells (YFV-venus) or BHK cells (DENV). The results are presented as plaque-forming units (PFU) per ml. g–j, Alphaviruses, such as Sindbis virus (SINV) and Ross River virus (RRV) are insensitive to SEC14L2 expression. Empty-vector- and SEC14L2-expressing Huh-7.5 cells were infected with SINV-GFP (g, h) or RRV-GFP (i, j) at an MOI of 0.05. The cells were harvested at the indicated times post-infection and FACS analysis was carried out to determine the number of green cells and the mean fluorescence intensities. All results in this figure represent mean ± s.e.m. of two replicate experiments.