Extended Data Figure 10: VE increased SEC14L2-mediated replicon colony formation.

a, VE (α-tocopherol, 1 µM) was added to Huh-7.5 cells 20 h before transfection with the indicated wild-type HCV subgenomic replicons. Transfections were carried out for 6 h in the fresh medium lacking VE, followed by medium change to VE-containing medium. After 48 h, cells were subjected to G418 selection and fed with fresh G418 and VE every 2 days. The resulting cell colonies were stained with crystal violet. Shown are the results of one of three independent experiments. b, Colony formation efficiency of the indicated subgenomic replicons was measured in SEC14L2-expressing cells in the absence or presence of 1 µM VE. The results are plotted as mean ± s.e.m. of CFU per 100,000 transfected cells from three independent transfections. **P < 0.005 by two-tailed, paired t-test.