Extended Data Figure 8: Orientation-biased joining of I-SceI DSBs at Sγ1 to AID-induced S-region breaks in various DSBR-deficient backgrounds.

a, Schematic illustration of the ΔSγ1^2×I allele and joining outcomes from 3′-broken end (red arrow) to AID-initiated upstream Sμ and downstream Sε DSBs. b, Linear distribution of junctions between ΔSγ1^2×I 3′-broken end to AID-induced Sμ/Sε region DSBs in anti-CD40/IL4-stimulated wild-type (b, n = 4), ATM^−/− (c, n = 3), H2AX^−/− (d, n = 3) and 53BP1^−/− (e, n = 4) cells across the 200-kb Igh region (left panels), 10-kb Sμ (middle panels) and Sε (right panels). f, Bar graphs (average ± s.d.) showing the percentage of junctions mapped at ΔSγ1^2×I (break site), Sμ and Sε over the total number of junctions in the 200-kb Igh constant region in different genotypes. g, Bar graphs (average ± s.d.) showing the percentage of junctions from above various genotypes of ΔSγ1^2×I 3′-broken end libraries mapped to Sμ and Sε as average ± s.d. h, Bar graph (average ± s.d.) showing comparison of percentages of junctions cloned using ΔSγ1^2×I 3′-broken end involving resection of Sμ (top panel) and Sε (bottom panel) breaks in indicated backgrounds. For detailed legends and further discussion refer to the Supplementary Information.