Extended Data Figure 9: Inhibition of resection in 53BP1-deficient B cells by an ATM inhibitor (ATMi) does not rescue directional CSR joining to Sγ1.
From: Orientation-specific joining of AID-initiated DNA breaks promotes antibody class switching
a–d, Linear plots of pooled junctions across the 200-kb Igh constant region (left panels), the 20-kb region flanking downstream Sγ1 (middle panels) and Sε (right panels) from wild type plus DMSO control (a, n = 2), wild type plus ATMi (b, n = 3), 53BP1−/− plus DMSO (c, n = 3) and 53BP1−/− plus ATMi (d, n = 3) libraries are shown as above. e, Bar graph showing the percentage of Sμ, Sγ1 and Sε junctions (average ± s.d.) from wild type plus DMSO 5′Sμ libraries (left) and wild type plus ATMi 5′-broken end libraries (right, n = 3). f, Bar graph showing the percentage of Sμ, Sγ1 and Sε junctions (average ± s.d.) from 53BP1−/− plus DMSO (left) and 53BP1−/− plus ATMi (right) 5′Sμ libraries. For detailed legends and further discussion refer to the Supplementary Information.
