Extended Data Figure 1: Deletional CSR in in vitro activated B cells by DC-PCR; I-Sce1 DSBs within the Igh constant region locus in activated B cells join with orientation-independence.
From: Orientation-specific joining of AID-initiated DNA breaks promotes antibody class switching
a, Schematic representation of DC–PCR assay. b, DC–PCR results from anti-CD40/IL4-activated wild-type and 53BP1−/− B cells. c, Schematic representation of the HTGTS method. d, e, HTGTS libraries analyses of anti-CD40/IL4-stimulated IghI-96k B cells with 3′-broken end (d, red arrow, n = 3) or 5′-broken end (e, blue arrow, n = 3) primers. BE, broken end. f, HTGTS libraries with 5′-broken end primer (blue arrow, n = 3) from ΔSμ2×I/ΔSγ12×I B cells stimulated with anti-CD40/IL4. g, Bar graph depicting deletion:inversion and excision-circle:inversion ratios between two I-SceI sites and between I-SceI and S region in wild-type versus 53BP1−/− backgrounds. For detailed legends and further discussion, refer to the Supplementary Information.
