Extended Data Figure 5: Orientation-biased joining between rSμ and AID-induced Sα DSBs in CSR-activated CH12F3 cells.
From: Orientation-specific joining of AID-initiated DNA breaks promotes antibody class switching
a, Diagram outlining potential junction outcomes from 5′ rSμ (red primer) or 3′ rSμ (blue primer) AID-initiated broken end junctions to AID-initiated DSBs in Sα upon anti-CD40, IL4 and TGFβ stimulation of ΔSμ CH12F3 cells. b, c, Top panel shows HTGTS libraries analyses of day 2 (b) and day 3 (c) stimulated CH12F3 (non-productive allele ΔSμ-Sα, productive allele ΔSμ) cells cloning from 5′-broken end rSμ (red primer, n = 3), whereas lower panel shows HTGTS libraries cloning from 3′-broken end rSμ (blue primer, n = 3). d, Bar graph shows percentage of junctions (average ± s.d.) for 5′-broken end and 3′-broken end libraries indicated in b and c. e, Bar graph with percentage of junctions (average ± s.d.) from rSμ libraries mapped to prey Sα in the deletion (DEL) or inversion (INV) for 5′-broken end libraries and in excision circle (EC) or inversion orientation for 3′-broken end libraries. For detailed legends and further discussion refer to the Supplementary Information.
