Extended Data Figure 1: KBTBD8 is a developmentally regulated CUL3 adaptor.
From: Cell-fate determination by ubiquitin-dependent regulation of translation
a, Gene expression analysis by microarray of hESCs differentiated into embryoid bodies (EB) for 6 days (n > 30,000 transcripts, mean of 3 biological replicates, analysis of variance (ANOVA) P value <0.05; blue, downregulated genes; red, upregulated genes). b, Expression analysis of all CRL substrate adaptors, including KBTBD8, with data derived from the experiment described above. c, Expression analysis of CUL3 adaptors during hESC differentiation into hEBs (blue, downregulation; yellow, upregulation). d, mRNA levels of pluripotency markers and KBTBD8 during hESC differentiation into EBs, as determined by qRT–PCR (mean of 3 technical replicates ± s.e.m.). e, Protein levels of KBTBD8 during hESC differentiation into EBs, as seen by western blot (OCT4, NANOG: pluripotency; PAX6: CNS precursors; TFAP2: neural crest marker). f, KBTBD8 is expressed in hESCs, but not in somatic cell lines, as determined by qRT–PCR (mean of 3 technical replicates ± s.e.m.). g, Abundance of KBTBD8 in H9 hESCs, D3 mESCs, or somatic cell lines was determined by western blot analysis. h, KBTBD8 expression is downregulated during mouse embryonic stem cell (mESC) differentiation into mouse embryoid bodies, as determined by qRT–PCR (mean of 3 technical replicates ± s.e.m.). i, KBTBD8 protein levels are reduced during mESC differentiation, as shown by western blot analysis.
