Extended Data Figure 6: The Yap-dependent regenerative program is activated in Lgr5+ ISCs.
From: Yap-dependent reprogramming of Lgr5+ stem cells drives intestinal regeneration and cancer
a, ISH showing induction of Areg (i–ii), Ereg (iii–iv), Msln (v–vi), Il33 (vii–viii), Clu (ix–x) and Yap protein expression (xi–xii) in intestinal epithelium of LatsΔ/Δ;Lats2Δ/Δ mice. b, Fluorescence ISH to detect Edn1, Olfm4 and cryptdin1 expression in irradiated (2 dpi; 12 Gy) Yap+/Δ and YapΔ/Δ intestines (n = 3). Edn1 expression is detected in ISCs post-irradiation in a Yap-dependent manner. Arrowheads point to location of Olfm4+ ISCs. Images are representative of three stainings per gene performed on tissues derived from separate mice. Scale bars: a, 70 μm; b, 35 μm.
