Extended Data Figure 8: Role of Yap in Apc mutant cells.

a, Apc^Min/+ adenomas display high levels of nuclear Yap. b, Comparison of crypt proliferation in Yap^+/Δ;Taz^+/Δ;Apc^Δ/Δ and Yap^Δ/Δ;Taz^Δ/Δ;Apc^Δ/Δ mice at 2 and 4 days after tamoxifen injection by staining representative sections with Ki67 antibodies (panels i–iv). Sections in panels v and vi were immunostained with anti-Yap/Taz antibodies. Images are representative of three stainings performed on tissues derived from separate mice. Scale bars, 70 μm. c, d, Genotyping of tail and crypt DNA and western blot analysis from indicated mice at 4 days after tamoxifen injection to confirm deletion of Yap and Taz.