Extended Data Figure 10: Role of Yap in Apc mutant tumour initiating cells.

a, Experimental procedure to target Yap in Apc mutant Lgr5⁺ ISCs. b, Tracing of Apc^Δ/Δ cells by staining for the Wnt target gene Lef at 4 and 30 days after tamoxifen injection (dpi) of Yap;Apc^ΔLgr5-cre mice. Note Lef staining is absent in surrounding wild-type crypts. c, Consecutive sections from Yap;Apc^ΔLgr5-cre mice at 10 dpi demonstrate both Yap negative (open arrowheads) and Yap positive (filled arrowheads) Lef⁺ foci (panels i–iii). Arrowheads in panel iii indicate occasional nuclear Yap staining in early Lef⁺ foci. In Lef⁺ foci displaying aberrant crypt morphology, Yap is strongly nuclear (panels iv–vi). d, Consecutive sections from Yap;Apc^ΔLgr5-cre mice at 10 dpi showing the levels of caspase 3⁺ apoptotic cells in both Yap-positive (filled arrowheads, panels i–iv) and Yap-negative (open arrowheads, panels v–viii) Lef⁺ foci. e, Two sets of consecutive sections (i–iii and iv–vi) showing Lef, Yap and Lyz staining of representative Lef⁺ foci. f, Two sets of consecutive sections (i–vi and vii–xii) showing Lef, Yap and phospho-Egfr staining of tamoxifen-induced Yap;Apc^ΔLgr-cCre mice. Filled and open arrowheads indicate Yap-positive and -negative Lef⁺ foci, respectively and panels ii, iv, vi, viii, x and xii are enlargements of adjacent panels. Scale bars, 70 μm.