Extended Data Figure 2: Yap localization and function in organoid cultures.
From: Yap-dependent reprogramming of Lgr5+ stem cells drives intestinal regeneration and cancer
a, b, Crypts from Yap+/Δ and YapΔ/Δ mice were harvested and cultured under standard conditions (see Methods) for the indicated times. Panel b depicts the percentage of organoids showing 0, 1, 2, 3 or ≥4 de novo crypts at 4 days (error bars indicate s.e.m.; n = 7 (n represents the number of separate cultures per genotype per mouse; ***P = 0.0006, **P < 0.0021)). c, Proliferation and apoptosis in Yap+/Δ and YapΔ/Δ organoids grown for 3 days were evaluated by examining incorporation of Edu (red) and active caspase 3 (yellow), respectively. Endogenous Yap expression is shown in green. Panels iii and iii′ show cytoplasmic localization of Yap. Panels iv and iv′ show nuclear accumulation of Yap in forming crypts. Arrowheads indicate increased apoptotic cells in YapΔ/Δ organoids. Scale bars, 70 μm.
