Extended Data Figure 4: Lowering Rspo1 levels rescues growth of Yap mutant organoids.
From: Yap-dependent reprogramming of Lgr5+ stem cells drives intestinal regeneration and cancer
a, Fluorescence immunostaining of Yap+/Δ and YapΔ/Δ mice at 3 dpi to detect Ki67+ cells (yellow) and lysozyme positive (Lyz+) Paneth cells (red). b, Representative images of Yap+/Δ (i, iii, v) and YapΔ/Δ (ii, iv, vi) organoids cultured in reducing Rspo1 concentrations for 3 days. Scale bars: a, 35 μm and b, 70 μm. c, Quantification of the percentage of organoids displaying 0, 1, 2, 3 or ≥4 de novo crypts after 3 days in culture using indicated concentrations of Rspo1. Error bars indicate s.e.m.; n = 4 (n represents number of organoid cultures analysed per genotype per mouse). d, Relative expression of Paneth cell markers (Kit, Lyz, Wnt3, Defa5) was evaluated by qPCR. Graphs show representative results of 3 independent organoid cultures per genotype. Error bars represent minimal and maximal range in fold differences derived from the standard deviation in Ct values; n = 3 (n represents the number of technical repeats).
