Extended Data Figure 5: Human GSDMD processing.
From: Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling
a, Western blots of THP-1 extracts and supernatants at 3 h after LPS electroporation. b, Western blots of human GSDMD/HEK293T stable transfectants at 24 h after transient transfection of the indicated plasmids. A non-specific band is indicated with an asterisk. c, Cytotoxicity of the human proteins indicated at 24 h after transient transfection of HEK293T cells. Numbers indicate nanograms of plasmid transfected. Western blots (right) indicate protein expression. Expression of the N-terminal fragment (1–275) was below detection levels, presumably due to its potent toxicity. Graph shows mean ± s.d. of triplicate wells and represents three independent experiments. For source gels of a, b and c, see Supplementary Fig. 3.
