Extended Data Figure 7: Canonical inflammasome stimuli induce caspase-1-dependent processing of Gsdmd.
From: Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling
a, Western blots of BMDM extracts and supernatants at 8 h after stimulation. Cont, medium alone. LPS, LPS + Ctb. Asterisk indicates a non-specific band. b, Western blots of Gsdmd−/− BMDMs. Gsdmd−/−3 and Gsdmd−/−4 are independent knockout strains (Extended Data Fig. 8). c, IL-1β released from BMDMs. LPS, LPS electroporation. Graphs show mean ± s.d. of triplicate wells and represent three independent experiments. d, Immunoblots of Casp1−/− BMDMs stimulated with Pam3CSK4 for 5 h. For source gels of a, b and d, see Supplementary Fig. 3.
