Extended Data Figure 6: FOXO1 represses MYC signalling in ECs.
From: FOXO1 couples metabolic activity and growth state in the vascular endothelium
a, Microarray expression analysis of FOXO1 and of canonical FOXO target genes in AdFOXO1CA- and AdCTL-expressing HUVECs 16 h after transduction (n = 3). b, GSEA of the FOXO1 DNA-binding element (TTGTTTAC) gene set in AdFOXO1CA- or AdCTL-transduced ECs. ES, enrichment score; NES, normalized enrichment score. c, GSEA of MYC gene signatures39,40,41,42 showing the downregulation of MYC target genes in FOXO1CA-expressing HUVECs. d, qPCR expression analysis of MYC at 3, 6 and 16 h in AdCTL and AdFOXO1CA-transduced HUVECs (n ≥ 4). e, qPCR analysis (n = 4) of Myc mRNA levels in ECs isolated from Foxo1CA mice 24 h after transduction with a control or Cre (AdCre) adenovirus. f, Immunoblot analysis of MYC in ECs isolated from Foxo1CA mice following transduction with AdCTL or AdCre (n = 3). Cre-mediated recombination gave rise to a 2.8 ± 0.3-fold increase in FOXO1 protein expression. g, Expression analysis of MYC in HUVECs by western blotting after RNA interference (RNAi)-mediated knockdown of FOXO1 (siFOXO1). siSCR, scrambled control (n = 3). h, MYC protein expression in ECs isolated from Foxo1fl/fl mice 24 h after transduction with an AdCTL or AdCre-encoding adenovirus (n = 3). a, d–h, Data represent mean ± s.d., two-tailed unpaired t-test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
