Extended Data Figure 7: FOXO1 interferes with MYC signalling at different levels.
From: FOXO1 couples metabolic activity and growth state in the vascular endothelium
a, Western blot analysis of MYC, MXI1 and FBXW7 in AdCTL- or AdFOXO1CA–Flag-transduced HUVECs. b, Immunoblot analysis and quantification of MYC protein levels in AdCTL and AdFOXO1CA–Flag transduced HUVECs that were co-treated with the proteasomal inhibitor MG132 (n = 3). c, Analysis of MYC protein half-life in AdCTL- or AdFOXO1CA–Flag-transduced HUVECs. The day after transduction, HUVECs were treated with cylcoheximide (CHX) and incubated for the times indicated. Data represent mean ± s.d. Two-way ANOVA with Bonferroni’s multiple comparison post-hoc test. d, e, qPCR (d) and immunoblot analysis (e) of MYC levels in control (siSCR) or MXI1 (siMXI1) siRNA-transfected HUVECs that were also transduced with AdCTL or AdFOXO1CA–Flag (n ≥ 5). f, qPCR analysis of MYC target genes in siSCR or siMXI1-transfected HUVECs that were cotransduced with AdCTL or AdFOXO1CA (n ≥ 3). Data represent mean ± s.d. One-way ANOVA with Bonferroni’s multiple comparison post-hoc test was performed in b, d, e and f. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
