Extended Data Figure 8: MYC regulates genes involved in cell metabolism and growth in ECs.
From: FOXO1 couples metabolic activity and growth state in the vascular endothelium
a, b, Analysis of MYC expression by qPCR (a) and immunoblot (b) in scrambled (siSCR) and MYC (siMYC) siRNA-treated HUVECs 24 h after transfection (n = 7). c, GSEA of the MYC (CACGTG) DNA-binding element gene set in siSCR- or siMYC-transfected HUVECs. d, GSEA of MYC gene signatures39,40,41,42 showing the downregulation of MYC target genes in MYC-depleted HUVECs. e, Heat map of downregulated MYC signature genes in MYC-silenced HUVECs (n = 3). Genes highlighted in red indicate genes that are also suppressed by FOXO1CA overexpression. f, Table of KEGG gene sets enriched among genes downregulated in the MYC siRNA-transfected ECs. g, Expression analysis of FOXO1-regulated MYC target genes by qPCR in MYC-silenced HUVECs (n ≥ 4). a, g, Data represent mean ± s.d., two-tailed unpaired t-test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
