Extended Data Figure 1: Constitutive and inducible deletion of Foxo1 in ECs of mice.
From: FOXO1 couples metabolic activity and growth state in the vascular endothelium
a, Strategy to generate a conditional Foxo1 mutant allele in which exons 2 and 3 are flanked by lox sites. The structures of the genomic locus, the targeting vector, and the targeted allele are shown. FRT-Neo-FRT, neomycin resistance cassette flanked by FRT sites. TK1, thymidine kinase. b, Table of viable offspring from Tie2-cre;Foxo1fl/+ (male) and Foxo1fl/fl (female) intercrosses. c, Control (Foxo1fl/fl) and Foxo1EC-KO mutants (Tie2-cre;Foxo1fl/fl) at E10.5. d, PCR of genomic DNA from P5 control (Foxo1fl/fl, lanes 1 and 3) and Foxo1iEC-KO (Pdgfb-creERT;Foxo1fl/fl, lanes 2 and 4) pups untreated (lanes 1 and 2) or treated (lanes 3 and 4) with 4-OHT. Recombination of the floxed Foxo1 allele (Δ) occurs only in 4-OHT-injected animals that are Pdgfb-creERT2-positive. e, Immunofluorescence staining for FOXO1, VE-cadherin (VECAD) and isolectin-B4 (IB4) in a P5 mouse retina of 4-OHT-injected control and Foxo1iEC-KO mice. f, Confocal images of mTmG+ control- and Foxo1iEC-KOmice that were injected with 4-OHT from P1 to P4 and analysed for GFP, ERG and IB4 expression.
