Extended Data Figure 4: FOXO1 restricts EC propagation and vascular growth in a cell-autonomous manner.
From: FOXO1 couples metabolic activity and growth state in the vascular endothelium
a, Timeline for the analysis of angiogenesis in the embryonic hindbrain. Plug-positive female mice were injected with 4-OHT from E8.5 to 10.5 and embryos harvested on E11.5 for hindbrain dissection. b, Confocal images of E11.5 control and Foxo1iEC-CA hindbrains stained with IB4 and GFP. c, High-magnification images of IB4-stained blood vessels in the ventricular zone of control and Foxo1iEC-CA mice. d, Timeline for the analysis of control and Foxo1iEC-CA low-degree chimaeras that heterozygously co-express the mTmG Cre reporter. Control and Foxo1iEC-KO mice were injected with a single low dose of 4-OHT at P3 and retinas analysed at P5. e, f, Confocal images (e) and quantification (f) of control;mTmG and Foxo1iEC-CA;mTmG retinas after low-dose 4-OHT treatment at P3 (n = 9). Samples were labelled for GFP, ERG and IB4. Data represent mean ± s.d., two-tailed unpaired t-test. ****P < 0.0001.
