Extended Data Figure 8: Effect of VIVIT on WT HSCs.
From: Mitofusin 2 maintains haematopoietic stem cells with extensive lymphoid potential
a, Expression of lymphoid (Sox4 and Il7r) and myeloid/platelet (Vwf and Fli1) genes in HSCs treated with VIVIT or DMSO for 24 h. n = 3 biological replicates; *P < 0.05; Student’s t-test. b, Lymphoid/myeloid ratio of CD45.2+ HSCs cultured for 4 days in DMSO and 500 nM VIVIT, transplanted with 2 × 105 CD45.1+CD45.2+ competitor bone marrow cells into CD45.1+ recipients and analysed 15 weeks post-transplant for lymphoid/myeloid ratio of donor compartment. Plots, mean ± s.e.m.; n = 5–9 recipients from two transplant experiments; *P < 0.05; two-tailed Student’s t-test. c, Donor chimaerism analysis 15 weeks after transplantation of CD45.2+ HSCs transduced with IRES–GFP or VIVIT–GFP with 2 × 105 CD45.1+CD45.2+ competitor bone marrow cells into CD45.1+ recipients Plots, mean ± s.e.m.; n = 9–12 recipients from two independent transplant experiments; *P < 0.05; two-tailed Student’s t-test. Bottom panel shows lymphoid and myeloid donor contribution in each individual recipient.
