Extended Data Figure 1: Position of valine residues in the thermostabilized mutant TS-β1AR.
From: Backbone NMR reveals allosteric signal transduction networks in the β1-adrenergic receptor
a, Schematic representation of secondary structure and amino acid sequence of TS-β1AR. As compared to the wild type, the TS-β1AR has truncations at the N and C termini and the intracellular loop (ICL3) as well as nine thermostabilizing point mutations (colour coded green) and three additional point mutations (colour coded yellow). Valines labelled with 15N are indicated by bold circles for assigned (blue) and unassigned (grey) residues. b, Structure of β1AR in complex with carvedilol (PDB code 4AMJ). The protein backbone and carvedilol are shown in ribbon and red stick representation, respectively. The individual valines are depicted as spheres (blue, assigned; grey, not assigned) labelled by residue number. c, Full 1H–15N TROSY spectra of apo TS-β1AR and all investigated ligand complexes. The ligand chemical structures are shown as inserts. Resonances are marked with assignment information (black, firm; cyan, tentative).
