Extended Data Figure 4: Evidence for micro- to millisecond dynamics at the ligand entry/exit pathway.
From: Backbone NMR reveals allosteric signal transduction networks in the β1-adrenergic receptor
Left, region of the 1H–15N TROSYs showing the V314(6.59) 1H–15N resonance of TS-β1AR in the apo and various ligand-bound forms. The resonances of the apo and atenolol-bound forms are severely broadened in the 15N dimension indicative of backbone dynamics in the micro- to millisecond range. The broadening is not observed for the other more tightly binding ligands. The resonances for the low affinity ligands, atenolol, isoprenaline and dobutamine, and for the high affinity ligands, alprenolol, carvedilol and cyanopindolol cluster at different positions. Interestingly, the resonance of the apo form clusters with the high affinity ligands, indicative of a similar backbone conformation. Right, 15N and 1H line widths of the V314(6.59) resonance for the apo and ligand-bound forms of TS-β1AR.
