Extended Data Table 3 Summary of the NMR response to ligands, G protein activation and NB80 binding data for the original TS-β1AR construct and various reverse mutants towards the native β1AR sequence

From: Backbone NMR reveals allosteric signal transduction networks in the β1-adrenergic receptor

  1. *Determined from the shifts of the 1H–15N resonances in response to binding of atenolol and isoprenaline (see Extended Data Fig. 6B).
  2. †Determined from the Gi activation assay described in Extended Data Fig. 5.
  3. ‡Determined from NMR experiments by mixing of isoprenaline-activated TS-β1AR with NB80. The 1H–15N spectra are shown for TS-β1AR(A227Y/L343Y) in Extended Data Fig. 7. The spectra of the other TS-β1AR mutants showed no response to addition of NB80 up to equimolar concentrations of ~100 μM for both proteins. Values in parentheses show pIC50 for isoprenaline binding in the absence or presence of NB80, respectively (see Extended Data Fig. 7).
  4. §Melting temperature Tm of the apo form receptor determined by the CPM thermal shift assay.
  5. ||Native residues are highlighted in bold.
  6. ¶Not determined.
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