Extended Data Figure 7: Real-space orthogonal slices.
From: Macromolecular diffractive imaging using imperfect crystals
a–c, Slices of the observed structure factor (Fo) electron density map, plotted as a grey scale, of a single PSII dimer obtained from the 4.5-Å Bragg intensities following model refinement of that data. Each slice is one pixel thick (1.5 Å) and is normal to the z, y and x real-space axes, respectively (conjugate to qz, qy and qx). d–f, Slices through the 3D real-space support constraint used to for iterative phasing. The support was generated by blurring the 4.5-Å-resolution electron density map by 2.2 Å and then thresholding to achieve a binary mask. g–i, Slices through the 3.5-Å-resolution image obtained by iterative phasing of the continuous diffraction, using the support constraint illustrated in d–f and Fig. 3d.
