Extended Data Figure 7: RapaLink-1 is a more potent mTOR inhibitor than rapamycin.

a, MCF-7 cells were treated for 4 h with either RapaLink-1 (10 nM) or rapamycin (10 nM) with simultaneous addition of increasing doses of either rapamycin (left) or RapaLink-1 (right). Immunoblot analyses were performed on mTOR effectors. b, c, Mice bearing RR1 (b) or TKi-R (c) xenograft tumours were treated for 24 h with a single dose of either vehicle, rapamycin (10 mg kg⁻¹), AZD8055 (75 mg kg⁻¹) or RapaLink-1 (1.5 mg kg⁻¹) (n = 4 for each group). Immunoblot analyses were performed on mTOR effectors. d, MDA-MB-468 cells inducibly expressing mTOR wild type were treated with either rapamycin, MLN0128, a combination of rapamycin and MLN0128, or RapaLink-1 for 4 h. Immunoblot analyses were performed on mTOR effectors with the indicated antibodies. Rapamycin and MLN0128 panels are the same shown for wild type in Extended Data Fig. 2c and e, respectively.