Extended Data Figure 1: Acquired-mTOR mutations promote resistance to mTOR inhibitors in MCF-7 cells.
From: Overcoming mTOR resistance mutations with a new-generation mTOR inhibitor
a, The RNA from MCF-7 parental, RR1, RR2 and TKi-R cells was isolated and the polymerase chain reaction with reverse transcription (RT–PCR) products were submitted to Sanger sequencing at Genewiz. b, MCF-7 parental, RR1, RR2 and TKi-R cells were treated with either dimethylsulfoxide (DMSO) or 50 nM of RAD001 for 4 h. Immunoblot analyses were performed on mTOR effectors. c, d, MCF-7 parental, RR1, RR2 and TKi-R cells were treated with either DMSO as a control or 500 nM of either KU006, WY354 or PP242 mTOR inhibitors (c), or with different doses of MLN0128 (d) for 4 h. Immunoblot analyses were performed on mTOR effectors. All cellular experiments were repeated at least three times.
