Extended Data Figure 4: Purification of protein VII from infected cells.
From: A core viral protein binds host nucleosomes to sequester immune danger signals
a, Coomassie-stained SDS–PAGE analysis of fractions from RP-HPLC in Fig. 2a. The bands in fraction 38–41 min correspond to histone H1. Protein VII and V, as indicated, were verified by MS analysis (data not shown). The slight upward shift of the protein VII bands in the later peak corresponds to the higher abundance of protein preVII, as seen by HPLC in Fig. 2a. b, Western blot analysis of protein VII in HPLC fractions from a. c, Time course of infection followed by histone extraction and HPLC analysis. MS analysis verified peaks in each sample as indicated.
