Extended Data Figure 8: Differential PiCo and preBötC population responses to DAMGO and SST in horizontal and transverse slices.

a, After the application of 25 nM DAMGO, preBötC burst frequency decreases only slightly (n = 5), whereas PiCo bursting is nearly eliminated. b, Similar to results observed in horizontal slices, the PiCo rhythm is eliminated by 25 nM DAMGO in transverse slices that isolate PiCo and preBötC in the presence of 2 μM norepinephrine (n = 5). Periodic large amplitude bursts in the bottom preBötC trace are fictive sighs. c, DAMGO dose response of normalized preBötC and PiCo burst frequency in transverse slices, illustrating the differential sensitivity of the PiCo and preBötC rhythms to DAMGO; burst frequency values are normalized to baseline frequency in 2 μM norepinephrine (mean ± s.e.m., n = 8 with minimum replicates of 4 for each location and concentration). d, The PiCo rhythm is selectively and transiently inhibited by the application of 500 nM SST whereas the preBötC rhythm persists in horizontal slices. Graph shows normalized average burst frequencies of both rhythms at baseline, 1.5–3.5 min after SST application, and 8–10 min after SST application (n = 6). e, Similar to the horizontal slice, SST application results in a robust inhibition of PiCo bursting in paired transverse slices. f, Similar to d, complied normalized burst frequencies for n = 6 transverse slices before and after SST application. (mean ± s.e.m.) Two-way ANOVA followed by a Bonferroni post-hoc test. ****P < 0.0001 comparing PiCo to preBötC, ^ϕP < 0.05 compared to baseline.