Extended Data Figure 2: Norepinephrine dose response of preBötC and PiCo rhythms in horizontal and transverse slices.
From: A novel excitatory network for the control of breathing
The frequency of the PiCo rhythm (black) is highly sensitive to the application of low concentrations of norepinephrine while the preBötC rhythm (purple) stays relatively constant in both types of slice preparations. a, In horizontal slices the PiCo rhythm is slow under spontaneous conditions (n = 10), the two rhythms have similar burst frequencies in the presence of 2 μM norepinephrine (n = 6), and the PiCo rhythm significantly outpaces the preBötC rhythm under higher concentrations of norepinephrine (n = 4, 3–4 μM norepinephrine). b, Similarly, when isolated in transverse slices, the PiCo rhythm has a slow frequency under spontaneous conditions (n = 10), and the preBötC and PiCo have similar frequencies at 2 μM norepinephrine (n = 7; mean ± s.e.m.). Two-way ANOVA followed by a Bonferroni post-hoc test. ****P < 0.0001 comparing PiCo to preBötC, ϕP < 0.05 compared to baseline (Spon.), #P < 0.05 compared to 2 μM norepinephrine.
