Extended Data Figure 6: Optimization of the laser-ablation strategy.
From: A somitic contribution to the apical ectodermal ridge is essential for fin formation
a–l, Assessment of fluorescent recovery after laser ablation of Kaede-positive cells in the AER of double-transgenic Tg(tbx6:gal4-vp16); Tg(UAS:Kaede) embryos by scanning the region of interest (ROI) for 20 (a–c), 50 (d–f), 100 (g–i) and 200 (j–l) laser pulse iterations. m, Fluorescent intensity of the ROI before (scaled to 1, the initial fluorescence of the targeted cells) and after laser-mediated cell ablation. A very small amount of fluorescent recovery can be observed at 20 (c, m), 50 (f, m) and 100 (i, m) scan iterations, while 200 scan iterations results in a consistent and permanent loss of Kaede fluorescence (l, m). Scale bars, 10 μm.
