Extended Data Figure 2: Distally located GFP-positive cells in the AER of Tg(pax3a:GFP) transgenic larvae do not contribute to either vasculature or musculature.
From: A somitic contribution to the apical ectodermal ridge is essential for fin formation
a–c, Tg(pax3a:GFP) does not label vasculature in the zebrafish pectoral fin. Medio-lateral view of Tg(pax3a:GFP); Tg(kdr1:mCherry) pectoral fins at 44 hpf (a), 48 hpf (b), and 52 hpf (c). Elongated GFP-positive cells localize distally to the mCherry-positive cells of the developing vasculature (d–g). Tg(pax3a:GFP)-expressing cells in the AER do not colocalize with Tg(actc1b:mCherry) expression (d). Dorsal and ventral muscle masses are defined by mCherry expression (e). GFP and mCherry colocalization shown in white (f). Merged image (g). Arrows mark GFP-positive cells in the AER, while arrowheads mark the vasculature. Scale bars, 50 μm.
