Extended Data Figure 3: Validation of somite specific photoconversion of Tg(tbx6:gal4-vp16); Tg(UAS:Kaede) double-transgenic embryos. | Nature

Extended Data Figure 3: Validation of somite specific photoconversion of Tg(tbx6:gal4-vp16); Tg(UAS:Kaede) double-transgenic embryos.

From: A somitic contribution to the apical ectodermal ridge is essential for fin formation

Extended Data Figure 3

aq, Photoconversion of somite 4–7 (ak) and somite 4 (lq). a, Schematic representation of photoconversion. bd, Dorsal views after photoconversion of somite 4–7 at the 15 somite stage reveals non-photoconverted Kaede (green) (b) and photoconverted Kaede (red) (c). Merged image shown in d. Somites 1–7 are numbered. eg, Lateral views of the same photoconverted embryo at 2 dpf, revealing non-photoconverted Kaede (e) and a photoconverted Kaede (f). Merged image shown in g. h, Close-up view of photoconverted somites from eg including brightfield. ik, Close-up view of pectoral fin shown in eg showing non-photoconverted Kaede (i) and photoconverted Kaede (j). Merged image shown in k. Asterisks indicate presumptive fin muscle progenitors, arrows mark photoconverted Kaede-positive cells of the PHM (eg) and presumptive vasculature (ik), arrowheads indicate photoconverted Kaede-positive cells in the AER. ln, Dorsal view after photoconversion of somite 4 at 15 somites, showing non-photoconverted Kaede (l), photoconverted Kaede (m), and merged image (n). Somites 1–6 are numbered. oq, Lateral view of the same photoconverted embryo at 2 dpf, showing non-photoconverted Kaede (o), photoconverted Kaede specifically in somite 4 (p), and a merged image (q). Somites 2–6 are numbered. Scale bars, 100 μm (bg, iq), 50 μm (hk).

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