Extended Data Figure 4: Validation of homotopic somite transplantations.
From: A somitic contribution to the apical ectodermal ridge is essential for fin formation
a–d, Dorsal view of a somite 4 transplantation within a 13-somite stage embryo that has a Tg(ubi:zebrabow) (red) somite homotopically transplanted into a host embryo transgenic for Tg(actc1b:GFP), which marks the myotome in green. e–h, Dorsal view of the embryo in a–d at 24 hpf. i–l, Lateral view of the embryo in a–d at 2 dpf. m, Table of somite transplantations. Results from homotopic transplants of somites 3–6. n–p, Dorsal view of Tg(actc1b:GFP); Tg(pax3a:GFP) after somite 4 has been removed at the 15-somite stage. Solid lines indicate somite borders, numbers indicate somite identity. q–r, Lateral view of Tg(actc1b:GFP); Tg(pax3a:GFP) at 36 hpf. GFP-positive cells are present in the AER of both the control (q) and the experimental side (r) of the same embryo (n = 5). Scale bars, 100 μm (a–i), 50 μm (n–p) and 20 μm (q, r).
