Extended Data Figure 5: Optimization of MTZ-induced ablations.
From: A somitic contribution to the apical ectodermal ridge is essential for fin formation
a–d, Tg(tbx6:gal4-vp16); Tg(UAS-E1b:Eco.NfsB-mCherry) embryos were treated with 0, 20 and 40 mM MTZ from 30–48 hpf and analysed at 48 hpf. a, Initial analyses assessed muscle transgene expression patterns, embryonic survival and overall phenotype severity as well as the efficiency of ablation of mCherry-expressing cells within the AF. b–d, Antibody stain for mCherry at 48 hpf reveals disrupted morphology of mesoderm-derived cells in the AF. Medio-lateral view of pectoral fins at 48 hpf treated with 0 mM MTZ (n = 8) (b), 20 mM MTZ (n = 7) (c), and 40 mM MTZ (n = 11) (d). Arrows indicate dead or dying cells based on cell morphology and fragmentation, dashed lines indicate the border between the AF and underlying mesenchyme. Scale bars, 50 μm.
