Extended Data Figure 2: Mapping of a C-terminal region of eIF3d that protects the c-Jun 5′ cap structure.
From: eIF3d is an mRNA cap-binding protein that is required for specialized translation initiation
a, Validation of eIF3d subunit identification. eIF3d-cap crosslinking was validated by immunoprecipitation of eIF3d after crosslinking and denaturing the eIF3 complex by boiling in SDS. The result is representative of biological replicates. b, Limited proteolysis of eIF3 crosslinked to 32P-cap-labelled c-Jun 5′ UTR RNA. Full-length and proteolysis fragments of eIF3d are indicated by black and maroon arrows, respectively, on the phosphorimage and Coomassie-stained SDS gels. c, Mass spectrometry identification of trypsinized peptides from limited proteolysis of cap-crosslinked eIF3d. Identified peptides are highlighted in blue. The results in b and c are representative of three independent experiments.
