Extended Data Figure 3: Purification of eIF3d cap-binding domain.
From: eIF3d is an mRNA cap-binding protein that is required for specialized translation initiation
a, Alignment is coloured by phylogenetic conservation of amino acid physiochemical property similarity and a cartoon schematic of the eIF3d secondary structure is depicted below the sequences. Colouring begins at 30% conservation (lightest blue). b, Coomassie-blue-stained SDS gel of recombinant N. vitripennis eIF3d S172–F537 protein expressed in E. coli.
