Extended Data Figure 8: Effect of p53 deletion in the cellular dynamics of CPs and SCs.
From: Defining the clonal dynamics leading to mouse skin tumour initiation
a, Immunohistochemistry staining for p53 in Inv-CreER/Rosa-SmoM2 and K14-CreER/Rosa-SmoM2 clones 12 weeks after induction. b, Quantification of normal, hyperplastic, dysplastic and BCC clones in scale region of K14CreER/Rosa-SmoM2/p53fl/fl and Inv-CreER/Rosa-SmoM2/p53fl/fl mice. Description of number of counted clones is found in the Methods section. c, Distribution of clone sizes as measured by total cell content, imaged by confocal microscopy on whole mount tail epidermis. The number of clones analysed is indicated in Fig. 5d. Clone merger events were observed after 12 weeks following oncogenic activation in K14-CreER/Rosa-SmoM2/p53fl/fl preventing the accurate quantification of clonal persistence and clone size at longer times. d, Comparison of basal clone size distribution of Inv-CreER/Rosa-SmoM2/p53fl/fl versus Inv-CreER/Rosa-SmoM2 and K14-CreER/Rosa-SmoM2/p53fl/fl versus K14-CreER/Rosa-SmoM2 at 8 weeks and 12 weeks upon tamoxifen administration. e, Evolution of the clonal persistence of Inv-CreER/Rosa-SmoM2/p53fl/fl and K14-CreER/Rosa-SmoM2/p53fl/fl clones. f, Immunostaining of active-caspase-3 and SmoM2 8 weeks after induction in Inv-CreER/Rosa-SmoM2/p53fl/fl. g, Quantification of the proportion of apoptotic clones in Inv-CreER/Rosa-SmoM2/p53fl/fl (n = 90 clones from 3 independent experiments), and K14-CreER/Rosa-SmoM2/p53fl/fl (n = 82 animals from 3 independent experiments) 8 weeks after induction. Hoechst nuclear staining is represented in blue; scale bars, 10 μm. Error bars represent the s.e.m.
