Extended Data Figure 1: The fate of oncogene-targeted clones is determined by the initial targeted cell (SC or CP) and their location in scale or interscale regions.
From: Defining the clonal dynamics leading to mouse skin tumour initiation
a, Orthogonal view used to quantify the number of clones, cells stained with β4-integrin and SmoM2. (left). Quantification of the number of clones induced 1 week after tamoxifen administration in scale and interscale regions in K14-CreER/Rosa-SmoM2 (n = 4 animals, 0.1 mg tamoxifen) and Inv-CreER/Rosa-SmoM2 (n = 3 animals, 2.5 mg tamoxifen) (right). b, Immunostaining for β4-integrin and SmoM2 in K14-CreER/Rosa-SmoM2 and Inv-CreER/Rosa-SmoM2 clones located in the scale and interscale regions, 8 weeks after oncogene activation. c, Immunostaining for the differentiation marker keratin-10, K10, and SmoM2 in K14-CreER/Rosa-SmoM2 and Inv-CreER/Rosa-SmoM2 clones 8 weeks after oncogene activation, showing absence of differentiated cells in K14-CreER/Rosa-SmoM2 clones and alteration of the differentiation in Inv-CreER/Rosa-SmoM2 clones. Hoechst nuclear staining is represented in blue; scale bars, 10 μm.
