Extended Data Figure 1: Anatomy of the diagonal band of Broca.
From: A cholinergic basal forebrain feeding circuit modulates appetite suppression
a–c, The DBB is situated in the basal forebrain and consists of an anteriorly located vertical limb (VDB), which branches posteriorly into separate bilateral horizontal limbs (HDB). d, AAV-FLEX-tdTomato (pseudocoloured in green) injected bilaterally into the HDB to show expression throughout the full extent of the diagonal band (VDB and HDBs). e–g, Bilateral viral injections into the HDB (AAV-FLEX-Syn::EGFP shown as an example) are sufficient to target the full extent of the diagonal band (VDB and HDB). Scale bars, 100 μm. Schematic in a was adapted from images from the Allen Brain Institute Reference Atlas30.
