Extended Data Figure 5: Chaperome networks in type 1, type 2 and non-transformed cells.
From: The epichaperome is an integrated chaperome network that facilitates tumour survival
a, b, Heat maps illustrating all chaperome members (a) and the interactome of HSP90 (b) isolated by the HSP90 bait and identified upon mass spectrometry and bioinformatics analyses enriched (P < 0.1) in type 1 tumours over type 2 tumours and non-transformed cells. Protein sorting was based on hierarchical clustering. Last lane of the heat map in a shows the enrichment of these proteins on the HSP70 bait. c, Network illustrating the connectivity of proteins isolated by the HSP90 bait and identified upon mass spectrometry and bioinformatics analyses; chaperome members and proteins with scaffolding, adaptor, protein interface modulator roles and significantly enriched (P < 0.1) in type 1 tumours over type 2 tumours and non-transformed cells are shown. The thickness of the edges (connection lines) represents the robustness of the functional interaction. The colour of nodes represents protein abundance. For comparison, type 2 and non-transformed cells are also show. Core interactions are shown in Fig. 1f. d, e, The cargo or interacting proteins of HSP90 (d) and HSP70 (e) isolated by the PU-H71 and YK-chemical baits from the indicated cell homogenates. Protein levels in individual cell homogenates (input) were analysed by IEF and SDS–PAGE, as indicated. Proteins precipitated on the chemical bait were analysed by SDS–PAGE. Protein levels from each experimental condition were quantified and graphed (bottom). Data were repeated independently twice with representative data shown. f–i, Changes in multimeric chaperone complexes in cells challenged with multiple siRNAs against HSP90α or HSP90β (f), HSP90α/β, AHA1, HOP or HSP110 (g) and in cell homogenates challenged with antibodies specific for the indicated HSP70 paralogues and for HOP (h, i), as indicated. Levels of proteins in the homogenate were probed by SDS–PAGE or native-PAGE, as indicated. All data were repeated independently twice with representative data shown. For uncropped gels, see Supplementary Fig. 1.
