Extended Data Figure 5: Signal transduction by SRX-43.
From: Balancing selection shapes density-dependent foraging behaviour
a, Expression of Psrx-43::srx-43::SL2::GFP bicistronic reporter transgenes bearing N2 (top) or MY14 (bottom) srx-43 sequences. Arrows indicate cell bodies of ASI sensory neurons. Scale bars, 50 μm. b, ASH sensory neurons are insensitive to multiple ascarosides. ASH calcium imaging with GCaMP3 in control animals that do not express the srx-43 transgene, isolated as non-transgenic siblings of transgenic animals tested in Fig. 3f (n = 19). Ascarosides tested at 10 nM. c, SRX-43 from MY14 confers icas#9 sensitivity on ASH neurons. Compare SRX-43 from N2 in Fig. 3f. d, icas#9 decreases daf-7::GFP expression in ASI neurons of N2 but not roam-1MY14 adults. Bars indicate mean fluorescence intensity ± s.e.m. *P < 0.05 by ANOVA with Tukey’s multiple comparisons test. e, Responses to icas#9 of daf-7(lf) mutants are attenuated in N2 but not in roam-1MY14 genetic backgrounds. Modified exploration assays were conducted on strains including daf-3(lf) alleles (see methods). *P < 0.05 by t-test. Data presented as mean ± s.e.m. f, Time course for icas#9 response in exploration assay. Pheromone response expressed as mean ± s.e.m. for 2, 4, 6, 10, and 14 h following initiation of exploration assay. ***P < 0.001 by t-test with Bonferonni correction comparing squares entered in control plates versus 10 nm icas#9 plates; n = 12 for all time points.
