Extended Data Figure 7: MA-dependent NADPH oxidation by HODM in absence of formaldehyde production.
From: An oxidative N-demethylase reveals PAS transition from ubiquitous sensor to enzyme
The kinetic curve (mean values of initial rate plotted as a function of MA concentration) was fitted using the Michaelis–Menten function kcat = 7.0 ± 0.1 s−1 and KM = 0.55 ± 0.04 mM. Inset shows Purpald colourimetric assay for detection of substrate-dependent formaldehyde production. Data are represented as a percentage of expected formaldehyde produced for 1:1 conversion of DMA/MA to formaldehyde. Error bars represent s.d.
